kimA [2017-05-21 19:28:00]

high-affinity potassium transporter (uptake)

Locus: BSU04320

Isoelectric point: 9.93

Molecular weight: 66.60 kDa

Protein length: 607 aa

Gene length: 1821 bp

Function: potassium uptake

Product: high-affinity potassium transporter

Essential: no

Synonyms: ydaO

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Metal ion transporter
• Cellular processes → Homeostasis → Metal ion homeostasis (K, Na, Ca, Mg) → Potassium uptake/ export
• Information processing → Targets of second messengers → Targets of c-di-AMP
• Groups of genes → Membrane proteins

Gene

Coordinates: 486,432 → 488,255

Phenotypes of a mutant

• a kimA ktrA ktrB mutant requires high potassium concentrations on minimal medium with ammonium as nitrogen source PubMed

The protein

Catalyzed reaction/ biological activity

• uptake of potassium PubMed

Domains

• 11 trans-membrane helices at the N-terminus PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: kimA
  Description: PubMed

  Regulatory mechanism

  • ydaO riboswitch: termination/antitermination, expression is switched off upon binding of c-di-AMP (formed at high potassium concentration) PubMed, in ydaO riboswitch

  Regulation

  • expressed at low potassium concentration (ydaO riboswitch) PubMed

Biological materials

Mutant

• MGNA-C090 (kimA::erm), available at the NBRP B. subtilis, Japan
• 1A688 (kimA::erm), available at BGSC
• GP93 (kimA::cat), available in Jörg Stülke's lab PubMed
• JH642 and its derivatives: natural deletion of the 18 kb ydzA-mntH region encompassing the genes ydzA-topB-ydaJ-ydaK-ydaL-ydaM-ydaN-kimA-mutT-ydaP-ydzK-mntH PubMed

Expression vector

• pGP2913 (N-terminal His-tag, expression in E. coli, in pWH844), available in Jörg Stülke's lab

LacZ fusion

• BP144 (based on pAC5), available in Fabian Commichau' and Jörg Stülke's labs PubMed

FLAG-tag construct

• GP2405 ydaO-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab PubMed

Labs working on this gene/protein

• Jörg Stülke, Göttingen, Germany

References

Reviews

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Original publications

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